Effect of RNA Isolation Method on microRNA Quantity and Quality in Plasma: A Comparative Study
A preview of this application note (paraphrased from the introduction):
MicroRNAs (miRNAs) are small, non-protein coding RNAs that post-transcriptionally regulate gene expression by suppressing target mRNAs. It has recently been demonstrated that miRNAs circulate within blood in a highly stable, cell-free form and can be detected in plasma and serum. In addition, tumor cells have been shown to release miRNAs into the circulation, and profiles of miRNAs in plasma and serum have been found to be altered in cancer and other diseases. These results indicate the potential for using circulating miRNAs as blood-based markers for molecular diagnostics. The objective of this study is to compare two commercially available plasma miRNA kits based on miRNA recovery, consistency, and PCR inhibition.