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Comparison of Stool DNA Preservation Methods

Comparison of Stool DNA Preservation Methods and Their Impact on Gut Microbiome Diversity

A preview of this application note (paraphrased from the introduction):

Appropriate preservation and storage of stool samples is crucial in maintaining DNA fidelity and microbial community composition for downstream applications and analysis, including next-generation sequencing (NGS) and microbiome characterization. Post-collection bias may be introduced in cases where sample material is inadequately preserved, highlighting the importance of preservation in study design. This application note investigates differences in microbiota authenticity, DNA concentration, and DNA fidelity when stool is preserved by rapid freezing to -80°C, using Norgen Biotek’s Stool Preservative, or using TE buffer.

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