Soil Total RNA Purification Kits
For the rapid preparation of inhibitor-free total RNA from soil
The Soil Total RNA Purification Kit from Norgen Biotek provides a convenient and rapid way to purify total RNA from small amounts of soil samples. With this kit, all types of soil samples can be processed, including common soil samples and difficult soil samples with high humic acid content such as compost and manure.
The Soil Total RNA Purification Kit removes all traces of humic acid using the provided Bead Tubes along with a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify RNA of all sizes, from large mRNA and ribosomal RNA to microRNA and small interfering RNA.
The Soil Total RNA Purification kit protocol does not require the use of phenol or chloroform, thereby producing a user-friendly procedure and allowing for a higher throughput analysis. The purified RNA isolated with the Soil Total RNA Purification kit is of the highest integrity and can be used in a number of downstream applications including real time PCR and reverse transcription for gene expression analysis.
Product features
The Soil Total RNA Purification Kit can be used to isolate all RNA species from soil samples, including large mRNA and ribosomal RNA to microRNA (miRNA).
The Soil Total RNA Purification Kit can be used to process all types of soil samples, including common soil samples and difficult soil samples with high humic acid content such as compost and manure.
The Soil Total RNA Purification Kit removes all traces of humic acid using the provided Humic Acid Removal Columns and a combination of chemical and physical homogenization and lysis. This removes all brown color from the samples.
The purified RNA from the Soil Total RNA Purification kit is free from all inhibitors including humic acid, and can be used directly in a number of downstream applications like real-time PCR and reverse transcription PCR for gene expression analysis.
The Soil Total RNA Purification Kit has a rapid spin column format that allows for the isolation o RNA in under 30 minutes.
The Soil Total RNA Purification Kit includes: Lysis Buffer I, Binding Buffer E, Solution BX, Wash Solution A, Elution Solution A, Bead Tubes, Spin Columns, Humic Acid Removal Columns, Collection Tubes, Elution Tubes (1.7ml), product insert.
- Suggested soil input 500mg
- Maximum column loading volume 650µL
- Type of soil processed Common soil, compost, manure, etc.
- Time to complete 10 purifications 30 minutes
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