For rapid and simple sequential purification of circulating RNA & DNA from plasma & serum samples
The Plasma/Serum RNA/DNA Purification Mini Kit from Norgen Biotek provides a fast, easy, and reproducible way to sequentially isolate circulating RNA, exosomal RNA, and cell-free circulating DNA (cfc-DNA) from the same plasma/serum sample.
The Plasma/Serum Purification Kit is designed to sequentially isolate all sizes of circulating RNA (including microRNA), exosomal RNA, and cfc-DNA from fresh or frozen plasma/serum samples ranging from 10µL to 200µL. Unlike other kits on the market, Norgen’s Plasma/Serum Purification Kit does not require the use of phenol, chloroform, or any protease treatments to isolate the RNA or DNA from plasma/serum samples.
The purified RNA or DNA can be eluted into flexible elution volumes ranging from 10µL to 25µL, which can then be used in a number of sensitive downstream applications like qPCR, reverse transcription qPCR, methylation-sensitive PCR, reverse transcription PCR, NGS, Northern blotting, Souther Blot analysis, RNase protection and primer extension, and expression array assays.
The Plasma/Serum RNA/DNA Purification Mini Kit has a rapid spin-column format that allows for the processing of multiple samples in less than 20 minutes.
The Plasma/Serum RNA/DNA Purification Kit can isolate circulating DNA from 10µL to 200µL of plasma/serum.
Cell-free circulating DNA present in input volumes of 10µL to 400µL are concentrated into final elution volumes of 10µL to 50µL.
With the Plasma/Serum RNA/DNA Purification Kit, researches can use the purified RNA/DNA in a number of downstream applications like PCR, qPCR, methylation-sensitive PCR and Souther Blot analysis.
The Plasma/Serum RNA/DNA Purification Kit can be used to isolate all sizes of fragmented circulating RNA and exosomal RNA, including microRNA.
The Plasma/Serum RNA/DNA Purification Kit includes: Lysis Buffer A, Wash Solution A, Elution Solution A, Elution Buffer B, Micro Spin Columns, Collection Tubes, Elution Tubes (1.7ml), product insert.
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