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FAQ: Frozen Aliquotters

How long does the LN2 last when operating the CXT 353?

Once the instrument is up to temperature, you have a working time of 1 hour before the next fill. The refill time depends on the evaporation of the LN2. If your lab is warm and the humidity is higher, the LN2 evaporation will be faster. If your lab is colder and the humidity is lower, the LN2 evaporation will be slower. You do not need to refill the LN2 tank until the temperature read goes below -80C.

More from Frozen Aliquotters

No, the sample will not thaw inside the coring probe. Since both the probes and parent samples are kept at -80C, there is not enough time or  temperature difference to thaw the partial sample during extraction.

It takes approximately 1 minute to core a sample. This includes the time it takes to hit the core bottom and then deposit the core in the destination tube.

No. The serrated edges of the coring probe lose their sharpness after 1 core. This means that consecutive cores will become jagged and more difficult to eject.

The LN2 tank is a 5-liter tank – it holds up to 4 liters of LN2.

The CXT 353 can extract up to 5 frozen aliquots from a single 2.00ml vial.