Total RNA Purification Kit

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Total RNA Purification Kits

For rapid purification of total RNA—including microRNA—without phenol

Total RNA Purification Kit from Norgen Biotek

The Total RNA Purification Kit from Norgen Biotek lets researchers quickly isolate and purify the total RNA from cultured animal cells, tissue samples, blood, plasma, serum, bacteria, yeast, fungi, plants, and viruses. This kit can be used to purify all sizes of RNA from large mRNA and ribosomal RNA to microRNA (miRNA) and small interfering RNA (siRNA).

With the Total RNA Purification Kit, RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Product features

Diverse Isolation

The Total RNA Purification Kit isolates a range of RNA species, from large mRNA and ribosomal RNA to microRNA (miRNA) and small interfering RNA (siRNA).

Fast & easy processing

The Total RNA Purification Kit uses a rapid spin-column format that allows for the processing of 10 samples in 20 minutes.

Accuracy with small samples

Total RNA has been isolated and detected from as little as a single animal cell with the Total RNA Purification Kit from Norgen Biotek.

High quality DNA

The recovered RNA from the Total RNA Purification Kit is suitable for downstream applications like real-time PCR, Northern blotting, and expression array analysis requiring the use of intact RNA.

No Phenol or Chloroform

With the Total RNA Purification Kit, total RNA is isolated without the use of harmful chemicals such as phenol of chloroform.

Kit components

The Total RNA Purification Kit includes: Buffer RL, Wash Solution A, Elution Solution A, Mini Spin Columns, Collection Tubes, Elution Tubes (1.7ml), product insert

  • Column binding capacity 50µg
  • Maximum column loading volume 650µL
  • Size of RNA purified All sizes
  • Time to complete 10 purifications 20 minutes
  • Average RNA yield HeLa Cells (1 x 10^6 cells) 15µg
  • Average RNA yield E. coli (1 x 10^9 cells) 50µg
  • Average RNA yield S.cerevisiae (1 x 10^7 cells) 30µg
  • Storage conditions Keep all solutions tightly sealed at room temperature

Satisfied customers

That's why we do it

"At NBS Scientific I got quick and reliable responses to questions and appointments. All advice and attention was expertly given. We've been happily working together for over 2 years now. "

The Charles River Labs logo
Charles River Laboratories

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